Sartoclear Dynamics® is a new single-use technology for harvesting animal cell cultures with high cell densities. Consistent results, ease of use, enormous speed and flexible scalability are the key characteristics of this technology.
Inspired by the plasma industry, Sartoclear Dynamics® is based on the principles of body feed filtration. The ready-to-use clarification system enables single-use processing up to 2,000 liters. As a result, it replaces centrifuges and the subsequent depth filters in a single step.
In the past decade, the productivity of fed-batch mammalian cell cultures has risen significantly. Driven by optimized strains and culture media, biomass concentrations have increased from 1 – 3 g/l to 7 – 10 g/L.
High biomass concentrations challenge depth filters so that capacities have become very limited. Above 500 L a centrifuge seemed to be inevitable in order to reduce costs and waste.
With 2,000 L single-use bioreactors available a breakthrough technology was needed to enable a fully single-use process.
Sartoclear Dynamics® is a complete ready-to-use technology designed to harvest your bioreactor. Based on the principles of body feed filtration, it is ideal for high cell density cultures of up to 2,000 liters.
Our solution: The addition of a highly porous filter-aid to the feed stream. This increases the permeability of the filter cake and prevents the filter from getting blocked.
Our pressure vessels are manufactured in facilities certified for compliance with the European Pressure Equipment Directive 97/23/EC. Flexibility, thorough cleanability and ease of use are the major features of our pressure vessels. Sartorius Stedim Biotech pressure vessels are designed to fulfill our customers’ quality standards and thus completely meet market expectations. We can offer you a standard Tri-clamp connection as body closure but also an aseptic O-Ring connection similar to DIN 11864-3.
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With Sartoclear Dynamics®, the DE is directly mixed into the cell culture fluid. The innovative powder transfer system ensures a fast and dust-free transfer of the DE.
The powder transfer adapter is mounted on the seal.
The Sartoclear Dynamics® DE Powder Bag and the adapter are connected with a functional zipper.
Clamps keep the plastic film in position and protect the rails during powder transfer.
A large variety of different cell cultures were tested to find the best harvesting conditions for Sartoclear Dynamics® filters. The optimal conditions appeared to be constant for most cell lines.