Nitrocellulose membranes are the perfect choice for western blotting, DNA blotting as well as dot or slot
blots. Our nitrocellulose membranes are available in 0.22 μm and 0.45 μm pore size. They are
optimally adapted to all protein blotting systems such as electro transfer, semi-dry or simple capillary
blotting. The membrane can easily be cut to the desired gel dimension.
As nitrocellulose polymers are inherently hydrophobic, a characteristic which greatly contributes to
their very high protein binding capacity, the Sartorius Stedim Biotech membrane has been specifically
manufactured with hydrophilic reagents in order to allow the membrane to be wetted in a fast and
The 0.22 μm and 0.45 μm Nitrocellulose membranes show an optimal structure for blotting
applications. The small pore sizes allow for a high protein / DNA binding capacity onto the sponge like 3
dimensional structures. Up to 200 μg of IgG can be adsorbed in one cm2 of our Nitrocellulose
membrane. At the same time, the high pore ratio allows for a fast flow across the membrane. After
protein transfer, the nitrocellulose membrane can be blocked and rinsed according to usual protocols
followed by the required detection method.
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